###################### Anuncio #COVID19 #######################

Junto a colaboradores del Open Bioeconomy Lab, Open Science Network, FreeGenes y Ginkgo Bioworks, hemos creado una red de colaboración internacional y abierta, llamada ReClone, para sintetizar y distribuir ADN para la producción local de enzimas claves en reacciones de diagnóstico (>>Lista de reactivos<<). Este repositorio es de libre acceso e incluye vectores listos para expresión y purificación, así como una batería de componentes (e.g. tags, CDS, prom, etc) para armar diferentes combinaciones en respuesta a necesidades locales. En este link más detalles de la iniciativa. Por favor, ponte en contacto con nosotrxs si deseas ayudar!
En estos momentos estamos desarrollando un método de libre acceso de RT-qPCR (más info acá) y de RT-LAMP (protocolo de purificación BstLF acá y avances de la reacción <pronto acá>)

################################################################

Our group

We work at PUC (Chile), iBio Millennium Institute and CRG-FONDAP.  We promote open technologies for research and education in biology. We are part of the GOSH , ReClone, OpenPlant and TECNOx open technology communities.

Research interests:
Our lab focuses on two areas: i) Understanding and engineering spatial bacterial organization (e.g. Fractals, Tractable deformation) and ii) Open source technology for research, diagnostics and education (e.g. Open Fluorescent microscopy , LOOP/universal LOOP DNA fab tools). We use these resources in workshops and tech residencies (e.g. Bakubung report, open tech workshops,  UK-Africa GCRFLabfd-UST Mendoza; Comunicaciones Especulativas, reGOSH-CYTED and TReND in Africa) as well as collaborations on design and architecture (e.g. Synthetic Aesthetics, RedFungi).

Current open/collaborative projects (you are invited to join!):

    • ReClone: an international network from across the world seeking to support each other in accessing reagents for research and diagnostics, through sharing and partnership (soon web, forum, protocols)
    • CYTED Open Science Hardware development residencies (intensive three-week dev residency in Brasil_2019, Lima_2020, Mendoza_2021 & Chile_2022). More details at: Twitter, GitLab, Crowdfunding and Facebook
    • LightM with Alex Kutschera  – U. of Munich at https://github.com/vektorious/lightM
    • OpenVectors for uLOOP DNA assembly with Bernardo Pollak, Peter von Dassow & C. Dupont et al (JCVI) at https://osf.io/kw4fh/
    • RedFungi at https://redfungiblog.wordpress.com & Biofabrication lab. Open biofabrication protocols (fungi materials and devices of our collaborators at Biofab UC and LABVA)
    • Comunicaciones Especulativas project at http://interspecifics.cc/comunicacionesespeculativas/
    • Microscopes for yeast counting for beer brewers; with Nano Castro (GitLab) and wool fiber inspection (gitlab link soon).
    • Open hardware devices for isothermal reactions with Axel Sepulveda (repo link soon)
    • Biomaker Challenge (Web)
    • FluoPi v2: low cost device for whole plate fluorescence imaging (github)
    • Microscopes for exhibition setups (gitlab)
    • Open resources for teaching enzyme kinetics, PCR and LAMP at home (repo for code, and manual, BOMs and images at this OSF – work in progress)

Resources openly shared at:
Protocols.io (CC BY protocols)
SynBioUC and LabTecLibre Github(Code repos)
Open Science Framework (Projects data, notes and live updates)
Images under CC (BY-NC-SA 4.0) lab and personal collection

Contact details:
email: ffederici (at) bio (dot) puc (dot) cl

Universidad Catolica de Chile. Departamento Genética Molecular y Microbiología – Fac. Cs Biológicas.  Universidad Católica de Chile. Av Bernardo Ohiggins #340. Santiago. Chile
– OR –
Instituto de Ingeniería Biológica y Médica. 7mo piso Edificio Ciencia y Tecnología. Universidad Catolica de Chile. Campus San Joaquín. Avda. Vicuña Mackena 4860, Macul. Santiago, Chile

—————————    Free/Libre and Open Source Technology  ———————–

By Free/Libre and Open Source, we mean working practices and licenses that guarantee open access to methods, blueprints, documentation and resources as well as freedom to use, modify, sell* and redistribute technology. We apply these principles to the development of FLOS biological technology and scientific instrumentation in Latin America.

GOSH Gathering for Open Science Hardware 

The Global Open Science Hardware (GOSH) movement seeks to reduce barriers between diverse creators and users of scientific tools to support the pursuit and growth of knowledge.

Gosh photo album, Manifesto , gathering report and roadmap

Other links (if you want to read more about free/libre open source technology…):

Please check some of our workshops on open source technologies in the news tab.

(*)= open source technology can be commercialized (e.g. Opentrons, Sparkfun, openQCM,  openBCI, and more)

 

 

Gracias Diego…te vamos a extrañar toda la vida.